233 PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samp (Research paper – Ares-Arroyo, 2018)

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233 Research paper – Ares-Arroyo – 2018 – PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samp

In Significant Impact Groups: AMU reduction strategies \ Monitoring and surveillance; Disease/health
Species targeted: Pigs; Poultry; Sheep; Other;
Age: Young; Adult;
Summary:
Genes transferred by ColE1 plasmids are important vehicles for the spread of antibiotic resistance in two families of bacteria, Enterobacteriaceae and Pasteurellaceae. Their monitoring is essential, as they harbor important resistance elements that can lead to resistance to antimicrobials in bacteria found in humans, animals and the environment. Understanding how these genetic elements work can lead to better understanding of how resistance to antimicrobials is being spread. In this work we present a useful genetic tool for the detection and analysis of ColE1 plasmids, and confirm their important role in the dissemination of antibiotic resistance, especially in the Pasteurellaceae family of bacteria.

Where to find the original material: https://www.frontiersin.org/articles/10.3389/fmicb.2018.00469/full?report=reader; https://doi.org/10.3389/fmicb.2018.00469
Country: ES; IN

229 – The blaNDM-1-Carrying IncA C2 Plasmid Underlies Structural Alterations and Cointegrate Formation In Vivo (Research paper – Hadziabdic – 2019)

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229 Research paper – Hadziabdic – 2019 – The blaNDM-1-Carrying IncA C2 Plasmid Underlies Structural Alterations and Cointegr

In Significant Impact Groups: AMU reduction strategies \ Monitoring and surveillance Disease/health
Species targeted: Poultry;
Age: Not stated;
Summary:
Antimicrobial usage is most common trigger for the spread of antimicrobial resistance however reducing antibiotic use only is not sufficient to reverse resistance. Only eliminating antimicrobial selection pressure does not lead to plasmid loss in all plasmid/host combinations. In 2012, a type of Salmonella bacteria (Salmonella Corvallis) carrying a multi-resistance gene (blaNDM-1 plasmid) was detected in a wild bird in Germany. In recent broiler chicken infection study, we observed transfer of this genetic element to other bacteria. The stability of this gene transfer was observed in our in vivo study with broilers. Our study revealed most common structural alterations of this relevant gene were maintained in the experiments with the broiler flock. Results in this article indicate that for the future, reduction in antimicrobial usage must be combined with alternative approaches that target the loss of the resistance gene, in order to slow down the spread of resistance.

Where to find the original material: https://aac.asm.org/content/63/8/e00380-19; https://doi.org/10.1128/AAC.00380-19
Country: ES; IT; DE

225 Developing integrated livestock breeding and management strategies to improve animal health product quality and performance in European organic and low input milk (Industry Innovation)

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225 Industry Innovation – Developing integrated livestock breeding and management strategies to improve animal health product quality and performance in European organic and low input milk

In Significant Impact Groups: Breeding for disease resistance or robustness \ Housing and welfare
Species targeted: Pigs; Poultry; Dairy; Sheep;
Age: Adult;
Summary:
LowInputBreeds integrated breeding and management to improve animal health, product quality and performance in organic and low input milk, meat and eggs. Four technical sub-projects (SP) were evaluated for ethical, economic and environmental impact in a 5th SP, which also covered training and dissemination, including our website which lists contacts and bi-annual newsletters. By developing and integrating (a) genotypes selected for performance, robustness and product quality traits, and (b) management innovations to improve ‘low input’ systems the project made a significant contribution towards regionally-adapted breeding strategies, compatible with sustainable production, high product quality and organic principles.

Where to find the original material: https://cordis.europa.eu/docs/results/222/222623/final1-lib-publishable-summary-pdf.pdf;
Country: UK

221 Key actors in driving behavioural change in relation to on-farm biosecurity – A Northern Ireland perspective (Research paper – Lahuerta-Marin, 2018)

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221 Research paper – Lahuerta-Marin – 2018 – Key actors in driving behavioural change in relation to on-farm biosecurity_ a Northern Ireland perspective

In Significant Impact Groups: Biosecurity \Internal biosecurity; People
Species targeted: Pigs; Poultry; Dairy; Beef; Sheep; Other;
Age: Not stated;
Summary:
Agriculture and farming are valued contributors to local economy in Northern Ireland (NI). There is limited knowledge about farmers’ behaviours and attitudes towards disease biosecurity measures. As part of a larger project, a scenario-based workshop with key stakeholders was organised by the Agri-Food and Biosciences Institute (AFBI)-NI in December 2015. Participants were asked to discuss and give their opinions about a series of questions across four key areas in a semi-structured approach with an external facilitator. The discussion showed that training in biosecurity for farmers is important and necessary. Training was recommended to be provided by veterinary surgeons, preferably via a face-to-face format. The discussion addressing disease disclosure proved particularly challenging between those who were prospective buyers of cattle, and those who sold cattle. This workshop provided a unique and invaluable insight into key issues regarding farm level biosecurity activities.

Where to find the original material: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001042/; https://doi.org/10.1186/s13620-018-0125-1
Country: UK

214 Essential Oils in Drinking Water_ Using Flexibility and Speed to Help Poultry During Gut Health Challenges by Biomin (Industry Innovation)

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214 Industry Innovation – Essential Oils in Drinking Water_ Using Flexibility and Speed to Help Poultry During Gut Health Challenges by Biomin

In Significant Impact Groups: Feed / gut health \ Feed additives and supplements
Species targeted: Poultry;
Age: Adult;
Summary:
While adding phytogenic feed additives (PFAs) to feed is the most common application method, many producers are realizing the benefits of including PFAs in drinking water. PFAs can be used to tackle necrotic enteritis (NE) in poultry, a challenge likely to result in decreased feed intake. Bans on the use of antibiotic growth promotors in livestock production around the world have resulted in a performance gap. Phytogenic feed additives (PFAs) can help bridge the gap due to their beneficial biological effects. Drinking water application of PFAs (example of Digestarom® P.E.P. sol.) ensures they are delivered to the gut during times when they are most needed. Drinking water application of PFAs is flexible and quick and is increasing in popularity. Application can take place in combination with other additives such as organic acids, probiotics, or vaccines. Improved broiler gut quality and function results in reduced disease incidence and less treatment costs.

Where to find the original material: https://www.biomin.net/science-hub/essential-oils-in-drinking-water-using-flexibility-and-speed-to-help-poultry-during-gut-health-challenges-1/;
Country: IT; BE

213 Preventive Approach to Gut Health in Layers – feed additives Biotronic® and Digestarom® by Biomin (Industry Innovation)

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213 Industry Innovation – Preventive Approach to Gut Health in Layers – feed additives Biotronic® and Digestarom® by Biomin

In Significant Impact Groups: Feed / gut health \ Feed additives and supplements
Species targeted: Poultry;
Age: Adult;
Summary:
Even with good management, bacterial infections stemming from the imbalance of the gut microbiota can cause production losses in layers. Preventive nutrition strategies that support gut performance can offer a cost effective alternative to antibiotic application, particularly around peak production. In fact, unsaleable eggs and the challenge of drug-resistant bacteria can make antibiotic application more expensive than non-antibiotic alternatives. Organic acids and phytogenic feed additives have been accepted as an efficient tool to prevent and control disease proliferation in poultry. The case of a commercial layer farm in Hungary illustrates how preventive nutritional strategies using novel feed additives can offer a profitable alternative to antibiotics in cases of chicken disease.

Where to find the original material: https://www.biomin.net/science-hub/a-profitable-preventive-approach-to-gut-health-in-layers/;
Country: HU

209- Antimicrobial resistance programs in the European Union ( Research paper – Zorlescu – 2018)

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209 Research paper – Zorlescu – 2018 – Antimicrobial resistance programs in the European Union

In Significant Impact Groups: AMU reduction strategies \ Monitoring and surveillance Disease/health
Species targeted: Pigs; Poultry; Dairy; Beef; Sheep; Other;
Age: Not stated;
Summary:
At the European Commission (EC) level, legislation has been issued for the monitoring of antimicrobial resistance and these rules are applicable by each Member State (MS). For the proper implementation of the above legislation, audits are carried out in Member States that have developed programs on antimicrobial resistance that go beyond the EC’s requests. The aim of the study was the analysis of existing data reports, legislation and recommendations on antimicrobial resistance through which surveillance and monitoring is carried out in the European Union (EU). The audit reports issued between 2015 and 2017 by the Food Veterinary Office (FVO), as well as the articles issued by the EC through the antimicrobial resistance institutes were analysed. In conclusion, in order to improve antimicrobial resistance programs, a “good practice guide” can be achieved by MS with extensive experience in this area, to be used to harmonize antimicrobial resistance programs within the EU.

Where to find the original material: https://ojs.journals.cz/index.php/CBUIC/article/view/1281; https://doi.org/10.12955/cbup.v6.1281
Country: RO

208 – Bacterial resistance to antibiotics – a threat to humanity (Research paper – Draghici – 2017)

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208 Research paper – Draghici – 2017 – Bacterial resistance to antibiotics – a threat to humanity

In Significant Impact Groups: AMU reduction strategies \ Legislation and incentives Government
Species targeted: Pigs; Poultry; Dairy; Beef; Sheep; Other;
Age: Not stated;
Summary:
The concept of microbial resistance defines the ability of pathogens to survive and multiply in the presence of antibiotics. The resistant microorganisms are or become “tolerant” to antibiotics. The causes that led to this unprecedented situation are described here: releasing antimicrobials without prescription, unjustified prescription, failure to comply with the dosage, the use of antimicrobials with broad spectrum, prophylactic administration of antimicrobials, non-compliance with withdrawal periods after their administration to animals of economic interest, etc. The mechanisms of resistance occurrence are also described in this article: enzymatic inactivation, alteration or over-expression of the drug target, modification of metabolic pathways, reduced permeability or uptake, enhanced efflux. The role of International organizations such as OIE, WHO, FVO, HMA, EMA is also presented, along with a summary of the guidelines aiming to help national authorities of each state to develop its own strategies, leading to reducing the occurrence and spread of bacterial resistance.

Where to find the original material: https://agmv.ro/vol-27-nr-2-2017/;
Country: RO

207 – Evaluation of antimicrobial resistance in strains of E Coli isolated from broiler carcasses (Research paper – Lancu – 2018)

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207 Research paper – Iancu – 2018 – Evaluation of antimicrobial resistance in strains of e Coli isolated from broiler carcasses

In Significant Impact Groups: AMU reduction strategies \ Monitoring and surveillance Disease/health
Species targeted: Poultry;
Age: Not stated;
Summary:
Chicken products may be a source of infection with pathogenic Escherichia coli strains (ExPEC) and may present a zoonotic risk through multiple antibiotic resistances. The emergence of multiple antibiotic resistances (resistance to three or more classes of antimicrobial agents) among E. coli strains isolated from birds has created major economic and human health problems. A total of 30 strains of E. coli have been isolated and identified from fresh chicken carcasses harvested at different time intervals over a period of 12 months from different manufacturers. Strains identified as E. coli were tested on 12 antimicrobial substances and showed multiple resistances. The highest resistance was recorded at erythromycin and doxycycline (96.6%), and the smallest resistance was recorded at gentamicin 10%. E. coli strains with multiple antibiotic resistances are one of the main cause of infections in humans and birds.

Where to find the original material: https://agmv.ro/vol-28-nr-4-2018/; ISSN: 1220-3173
Country: RO

205 – The prevalence of salmonlla serotypes in domestic and wild animals and their spatial distribution in Romania between 2015 and 2016 (Research paper – Neghirla – 2018)

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205 Research paper – Neghirla – 2018 – The prevalence of salmonella serotypes in domestic and wild animals and their spatial distribution in Romania between 2015 and 2016

In Significant Impact Groups: AMU reduction strategies \ Monitoring and surveillance; Disease/health
Species targeted: Pigs; Poultry; Dairy; Beef; Sheep; Other;
Age: Young; Adult;
Summary:
Salmonella are major worldwide zoonotic pathogens infecting a wide range of animal species as well as humans. This study investigates the prevalence of circulating Salmonella serotypes in domestic and wild animal species in Romania and the spatial distribution of Salmonella isolates at county level in the period 2015-2016. There were collected 2551 samples for the isolation and serotyping of Salmonella according to ISO 6579:2002/Amd 1:2007 protocol. The findings of this study show that 36 Salmonella serotypes were isolated in 1357 Salmonella-positive samples in 2015, and 28 serotypes in 1188 Salmonella-positive samples in 2016. One sample from pigeon and one from hen were negative in 2015, and one sample from pigeon and three samples from hen were negative in 2016. In order to reduce the prevalence of serotypes with major zoonotic potential, we recommend continuing collaboration of all the professionals involved in the security of the food chain.

Where to find the original material: https://agmv.ro/vol-28-nr-4-2018/; ISSN: 1220-3173
Country: RO